We got to show off our new phenotyping systems, OCTOflux and the PARdots (which is also a good name for a band), at the University of Sydney Plant Breeding Institute (PBI) Narrabri Field Day this week. We also test-fired OCTOflux on wheat for the first time, and will make some final modifications next week. Thanks to Tam Salter for taking photos (click on the pictures for full-size images).
Progress on manufacturing PARdot handmade PPFD sensors. I’ve soldered 284 photodiodes to their wires and epoxied them to diffusers. Next stage: glue acrylic ring to underside of diffuser and fill with epoxy to waterproof. Field testing at Narrabri next week (weather permitting)!
Tam Salter has made great progress on the OCTOflux high-throughput field physiological phenotyping system for the IWYP project. When complete, the system will be able to measure photosynthesis and transpiration, and thus photosynthetic capacity, on eight leaves simultaneously, using a single-channel IRGA and a bank of solenoid valves to multiplex the sample and reference gas streams. The system will be operated by an Excel program on a laptop (or tablet), using VBA to interface with a DLL that talks to the MCCDAQ data acquisition and relay control boards. The entire system should cost roughly $20k. We’ll be able to complete approximately 32 Amax measurements per hour, enabling a massive step increase in throughput for field physiotyping of diverse germplasm for pre-breeding, or variation in leaf physiology for ecological studies.
We’ll post updates as progress continues, and when the system is dialed in we’ll post complete specifications and instructions for replicating the system.
Just returned from an epic trip to the US and France for a Gordon Conference on Multiscale Plant Vascular Biology and a New Phytologist workshop on the Kok effect (which I have christened the “Kokference”). I presented some cool new work on coupled heat and energy transport in leaves and got to hang out with a lot of really smart people! The Gordon Conference format is really great and conducive to progress — thanks for the organizers for inviting me!
I visited colleagues Matthew Gilbert and Heather Vice at UC Davis on the way back, to pick up instructions and machined parts to build Matthew’s amazing PARdot homemade ceptometers for the IWYP project.